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Literature summary extracted from
- Magnetic proteinase K reactor as a new tool for reproducible limited protein digestion (2008), Bioconjug. Chem., 19, 966-972.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.64 | additional information | highest activity using 3 mg of PK per mg of the carrier | Parengyodontium album |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.64 | medicine | PK magnetic reactor is a useful tool for prion protein digestion | Parengyodontium album |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.64 | additional information | immobilization of PK on magnetic latex microparticles (500A5 microparticles) and other chemically and structurally similar types of magnetic carriers | Parengyodontium album |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.64 | 1.29 | - |
succinyl-Ala-Ala-Ala-p-nitroanilide | immobilized PK | Parengyodontium album |  |
| 3.4.21.64 | 4.23 | - |
succinyl-Ala-Ala-Ala-p-nitroanilide | native PK | Parengyodontium album | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.64 | Parengyodontium album | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.64 | human growth hormone + H2O | proteolytic activity and specificity of PK is maintained after its immobilization to magnetic particles | Parengyodontium album | ? | - |
? | |
| 3.4.21.64 | additional information | digestion of mouse cell lysates overexpressing prion proteins | Parengyodontium album | ? | - |
? | |
| 3.4.21.64 | succinyl-Ala-Ala-Ala-p-nitroanilide + H2O | - |
Parengyodontium album | succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.64 | Proteinase K | - |
Parengyodontium album |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.64 | 25 | 50 | soluble and immobilized enzyme | Parengyodontium album |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.64 | Parengyodontium album | - |
- |
8.4 |
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Release 2023.1 (January 2023)
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